Adameyko Lab
Medical University of Vienna
Karolinska Institutet
Aims of research
We are known for running projects that tackle different aspects of organismal biology. We look at a range of live systems from a mechanistic point of view, trying to reverse engineer different aspects of multicellular life. Tracing the incremental advancements in development of multicellular organisms from a single cell perspective allows better understanding of the complexity of the entire organism or organ system in a final phase. That is why our main strength is developmental biology. The knowledge gained from developmental biology research is widely applied in regenerative medicine. Thus, we hope to improve human health via discovering new fundamental ideas about how development, stem cells, and regeneration work.
Our laboratory advances a broad spectrum of projects related to developmental biology, stem cells, EvoDevo and regenerative medicine. The methodology includes classical developmental biology approaches blended with single cell transcriptomics, 2D sequencing and 3D-reconstructions of tissues and organs based on optical or X-ray methods (micro-CT, synchrotron).
The neural crest stem cells is our primary model system, where we address general principles of cell fate choice, transcriptional and epigenetic control of a lineage progression, morphogenesis, and tissue shaping.
Lab competences
Developmental studies
Lineage tracing
Cell type ablation
Cell selection via FACS
Advanced imaging
RNAscope
MERFISH (coming soon)
Slide-seq (coming soon)
Advanced scRNAseq analysis
Dataset clustering and integration
Trajectory analysis
Non-model organism exploration
Projects
*Featured Publications
Spatiotemporal structure of cell fate decisions in murine neural crest
Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single-cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial coactivation of bipotential properties followed by gradual shifts toward commitment. Competing fate programs are coactivated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.
Recent Publications
Prototypical pacemaker neurons interact with the resident microbiota
Proceedings of the National Academy of Sciences
Nerve-associated neural crest: peripheral glial cells generate multiple fates in the body
Current Opinion in Genetics and Development
Meet the Team
Head of the Lab
Postdoctoral fellows
PhD Students
Adameyko Lab
Medical University of Vienna
Karolinska Institutet
Aims of research
We are known for running projects that tackle different aspects of organismal biology. We look at a range of live systems from a mechanistic point of view, trying to reverse engineer different aspects of multicellular life. Tracing the incremental advancements in development of multicellular organisms from a single cell perspective allows better understanding of the complexity of the entire organism or organ system in a final phase. That is why our main strength is developmental biology. The knowledge gained from developmental biology research is widely applied in regenerative medicine. Thus, we hope to improve human health via discovering new fundamental ideas about how development, stem cells, and regeneration work.
Our laboratory advances a broad spectrum of projects related to developmental biology, stem cells, EvoDevo and regenerative medicine. The methodology includes classical developmental biology approaches blended with single cell transcriptomics, 2D sequencing and 3D-reconstructions of tissues and organs based on optical or X-ray methods (micro-CT, synchrotron).
The neural crest stem cells is our primary model system, where we address general principles of cell fate choice, transcriptional and epigenetic control of a lineage progression, morphogenesis, and tissue shaping.
Lab competences
Developmental studies
Lineage tracing
Cell type ablation
Cell selection via FACS
Advanced imaging
RNAscope
MERFISH (coming soon)
Slide-seq (coming soon)
Advanced scRNAseq analysis
Dataset clustering and integration
Trajectory analysis
Non-model organism exploration
Projects
Featured Publications
Spatiotemporal structure of cell fate decisions in murine neural crest
Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single-cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial coactivation of bipotential properties followed by gradual shifts toward commitment. Competing fate programs are coactivated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.
